Light-Controlled Expression of .BETA.-Glucuronidase Gene Driven by palg2b Promoter from Populus kitakamiensis in Nicotiana plumbaginifolia.
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A choline monooxygenase gene promoter from Salicornia europaea increases expression of the beta-glucuronidase gene under abiotic stresses in tobacco (Nicotiana tabacum L.).
A 1312 bp 5' flanking region of Salicornia europaea choline monooxygenase (SeCMO) gene was isolated using the anchored PCR. To investigate the mechanism of regulation for this stress-induced gene, the SeCMO promoter-beta-glucuronidase (GUS) chimeric gene constructs containing five deletions F1, F2, F3, F4 and F5 were introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium-mediated trans...
متن کاملCytokinins and auxins control the expression of a gene in Nicotiana plumbaginifolia cells by feedback regulation.
Both cytokinin (N6-benzyladenine [BA]) and auxin (2,4-dichlorophenoxyacetic acid [2,4-D]) stimulate the accumulation of an mRNA, represented by the cDNA pLS216, in Nicotiana plumbaginifolia suspension culture cells. The kinetics of RNA accumulation were different for the two hormones; however, the response to both was transient, and the magnitude of the response was dose dependent. Runoff trans...
متن کاملSequence of a Nicotiana plumbaginifolia beta(1,3)-glucanase gene encoding a vacuolar isoform.
/3(l,3)-glucanases are a group of hormonally and developmentaUy regulated hydrolytic plant enzymes, which are also induced upon pathogen infection. A cDNA and genomic clone of the Nicotiana plumbaginifolia /3(l,3)-glucanase gnl gene have been analyzed previously (1, 2). Here, we report the nucleotide and deduced amino acid sequences of a different N. plumbaginifolia 0(1,3)glucanase gene designa...
متن کاملCloning of an ovule specific promoter from Arabidopsis thaliana and expression of beta-glucuronidase.
Tissue specific expression of transgenes in plant species has several advantages over constitutive expression. Identification of ovule specific promoters would be useful in genetic engineering of plants with a variety of desirable traits such as genetically engineered parthenocarpy, female sterile plants or seedless fruits. Relative inaccessibility and difficulty in harvesting adequate amounts ...
متن کاملAnalysis of the potential promoter sequences of African cassava mosaic virus by transient expression of the beta-glucuronidase gene.
DNA fragments from promoter regions of the geminivirus, African cassava mosaic virus, were cloned into pG1, a vector based on pUC18, producing transcriptional fusions with the beta-glucuronidase (GUS) gene and nopaline synthase termination sequence. The activity of each promoter construct was assessed by analysing the transient expression of GUS in Nicotiana clevelandii protoplasts. The results...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2001
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.18.67